Seeding retrovirally transduced vascular smooth muscle cells onto denuded arteries achieves long-term, potentially therapeutic gene expression in a rat model, demonstrating a viable approach for gene therapy.
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Supports cell-seeding gene delivery in rat arteries; leaves open clinical translation pending human studies.
Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli beta-galactosidase gene or a human adenosine deaminase (adenosine aminohydrolase, EC 3.5.4.4) gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.
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Lynch et al. (1992) studied this question.
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