Key result
A constant temperature perfusion system was developed to heat perfusion fluid to 37 +/- 10 -4 degrees C for precision heat flow measurements on isolated working rat hearts.
The development of a highly stabilized constant temperature perfusion system enables precise myocardial energetics and heat flow measurements in isolated working hearts.
Answers are grounded in this paper and cite their sources.
Facilitates precise myocardial energetics research in isolated hearts; leaves open translation to clinical settings.
A constant temperature perfusion system employing four heat exchangers has been developed in which perfusion fluid is heated from room temperature to 37 +/- 10 -4 degrees C for precision heat flow measurements on isolated working rat hearts. The temperature characteristics have been established and mathematical expressions developed to identify and quantify spurious thermal events. The system is a refinement of existing perfusion systems for metabolic and mechanical investigations which meets the complete requirements of myocardial energetics. It can also be used for experiments which include high precision temperature measurements on isolated working hearts or for thermal investigations on other isolated perfused organs where a highly stabilised temperature base line is required over perfusion flows from 0-100 cm3 min -1.
No takes yet. Share an insight, caveat, or question.
Niesler et al. (1981) studied this question. Constant temperature perfusion system was evaluated on Temperature characteristics and precision heat flow measurements. A constant temperature perfusion system was developed to heat perfusion fluid to 37 +/- 10 -4 degrees C for precision heat flow measurements on isolated working rat hearts.
Synapse has enriched 10 related papers. Consider them for comparative context: