Key result
AT2 receptors were immunohistochemically detected in vasopressinergic neurons of the rat hypothalamus and posterior pituitary, but no AT2 binding was detected using receptor autoradiography.
Answers are grounded in this paper and cite their sources.
This preclinical study demonstrates the presence of AT2 receptor immunoreactivity in rat vasopressinergic neurons and the posterior pituitary, though functional binding at neurohypophyseal terminals remains unconfirmed.
Previous functional studies indicated that an angiotensin type 2 (AT2) receptor subtype may participate in the regulation of vasopressin release by angiotensin II (AngII). In the present study, AT2 receptor-directed antiserum immunohistochemically detected AT2 receptors within the hypothalamic paraventricular (PVN) and the supraoptic nuclei (SON) of the rat brain, more specifically, in identified vasopressinergic neurons. Considering the lack of AT2 binding in the PVN and the SON using receptor autoradiography, we tested the hypothesis that these AT2 receptors are transported to the posterior pituitary. Western blot analysis detected AT2 immunoreactivity in the posterior pituitary. However, no AT2 binding was detected in posterior pituitary membranes, and no AT2 binding was detected with quantitative receptor autoradiography in the neurohypophysis. Thus, if AT2 receptors are transported from the magnocellular vasopressin neurons to the posterior pituitary, their role in AngII regulation of vasopressin release at the neurohypophyseal terminals remains to be clarified.
No takes yet. Share an insight, caveat, or question.
Shelat et al. (1998) studied this question. AT2 receptor-directed antiserum immunohistochemistry and autoradiography was evaluated on AT2 receptor detection and binding in the hypothalamus and posterior pituitary. AT2 receptors were immunohistochemically detected in vasopressinergic neurons of the rat hypothalamus and posterior pituitary, but no AT2 binding was detected using receptor autoradiography.
Synapse has enriched 10 related papers. Consider them for comparative context: