Key result
Expression of bovine IL-12 as a self-processing polypeptide using a 2A peptide from FMDV resulted in successful cleavage into separate subunits and produced a functional heterodimer.
The use of a 2A peptide from FMDV allows for the coordinated and stoichiometric expression of complex heterodimeric proteins like IL-12 as a self-processing polypeptide.
Supports 2A peptide strategy for bovine IL-12 in preclinical models; leaves open translation to human therapeutics.
Interleukin-12 (IL-12) is a heterodimeric cytokine composed of two disulfide-linked subunits (p40 and p35) encoded by separate genes. We used the apparent autocleavage property of a 2A peptide from the foot-and-mouth disease virus (FMDV) to express bovine (Bo) IL-12 as a self-processing polypeptide (p402Ap35). We demonstrate that 2A will mediate the cleavage of p402Ap35 into two separate subunits in a manner similar to that observed during the processing of the FMDV polypeptide. Furthermore, this 2A polypeptide encoded a functional heterodimer, which elicited activities associated with IL-12 in other species. We propose that this strategy of self-processing polypeptides may be used in many applications where the coordinated and stoichiometric expression of complex proteins is required.
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Chaplin et al. (1999) studied this question. Self-processing 2A polypeptide strategy for bovine IL-12 expression was evaluated on Cleavage of p402Ap35 into two separate subunits and functional heterodimer activity. Expression of bovine IL-12 as a self-processing polypeptide using a 2A peptide from FMDV resulted in successful cleavage into separate subunits and produced a functional heterodimer.
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