What question did this study set out to answer?

To elucidate the biological function, tissue distribution, and glycan-binding specificity of the C-type lectin CLEC4F in the liver.

June 6, 2013PLoS ONEOpen Access

CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver

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Authors

CYChih-Ya YangNational Yang Ming Chiao Tung University JCJiun-Bo ChenKing's College London TTTing‐Fen TsaiNational Yang Ming Chiao Tung University

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Implication

Experimental study demonstrates that CLEC4F facilitates alpha-galactosylceramide presentation by Kupffer cells in murine liver, indicating an inducible lectin role in hepatic immune regulation.

Key Points

To elucidate the biological function, tissue distribution, and glycan-binding specificity of the C-type lectin CLEC4F in the liver.
Characterized murine CLEC4F developmental expression and distribution in fetal and adult livers, as well as during Listeria monocytogenes infection using wild-type and Clec4f-knockout (Clec4f-/-) mice.
Profiled carbohydrate-binding affinity across diverse carbohydrate structures via glycan array screening.
Assessed calcium-dependent binding to alpha-galactosylceramide (α-GalCer) and evaluated its presentation to natural killer T (NKT) cells.
CLEC4F is a heavily glycosylated membrane protein co-expressed with F4/80 on resident Kupffer cells from embryonic day 11.5 and is induced on infiltrating mononuclear cells during Listeria monocytogenes infection.
Glycan array profiling showed strong binding to galactose and N-acetylgalactosamine, whereas terminal fucosylation selectively blocks CLEC4F recognition.
CLEC4F directly binds alpha-galactosylceramide in a calcium-dependent manner and participates in presenting this glycolipid to natural killer T cells.

Cite This Study

Yang et al. (2013) studied this question.

synapsesocial.com/papers/6a92e059fdc099c5d297cb1f https://doi.org/10.1371/journal.pone.0065070

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Implication

Experimental study demonstrates that CLEC4F facilitates alpha-galactosylceramide presentation by Kupffer cells in murine liver, indicating an inducible lectin role in hepatic immune regulation.

CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver

Discussion

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Implication

Key Points

Cite This Study

CLEC4F Is an Inducible C-Type Lectin in F4/80-Positive Cells and Is Involved in Alpha-Galactosylceramide Presentation in Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

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