The Antibiotic Neomycin Enhances Coxsackievirus Plaque Formation

Figures

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  FIG 1. Effect of neomycin on plaque formation of CVB3-Nancy, poliovirus, and reovirus. (A) Effects of antibiotics on CVB3-Nancy plaque formation. HeLa cells were pretreated with or without 1 mg/ml of the indicated antibiotics prior to plating of 100 PFU of CVB3-Nancy on cells with agar overlays with or without 1 mg/ml of each antibiotic. Plates were stained with crystal violet. Abx, ampicillin, neomycin, streptomycin, and vancomycin mixture. (B) Effect of neomycin on CVB3-Nancy plaque formation. The experiment was performed as for panel A, in the presence or absence of neomycin at various concentrations. (C and D) Effects of neomycin on poliovirus (C) or type 3 Dearing reovirus (D). (E) Plaque size quantification of CVB3-Nancy, poliovirus, and reovirus in the presence or absence of 1 mg/ml of neomycin. Each symbol represents a plaque. ****, P < 0.0001 (unpaired two-tailed Student t tests). ns, not significant. All images are representative of results from at least 2 or 3 independent experiments.

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  FIG 2. Effect of neomycin on plaque formation of different CVB3 strains. HeLa cells were pretreated or not with neomycin and were infected with approximately 100 PFU of CVB3-Nancy, CVB3-Nancy-N63Y, or CVB3-H3, and then agar or agarose overlays, containing or lacking 1 mg/ml of neomycin, were added. All images are representative of results from at least two independent experiments.

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  FIG 3. Effects of neomycin on early stages of the CVB3-Nancy replication cycle. (A) Cell attachment assay. A total of 1 × 106 HeLa cells were pretreated or not with neomycin prior to incubation with 6,000 cpm (6 × 106 PFU) of 35S-labeled CVB3-Nancy or no virus (mock) at 4°C for 20 min to promote viral binding. Cells were washed and 35S was quantified in a scintillation counter. Data are means ± SEMs (unpaired two-tailed Student t tests; n = 5 total data points from two independent experiments). (B) Viral protein synthesis assay. A total of 2.5 × 106 HeLa cells that had been pretreated or not with neomycin were inoculated with CVB3-Nancy at an MOI of 20 for 30 min at 37°C. At the indicated time points, cells were washed and incubated in 1 ml of DMEM lacking methionine and cysteine supplemented with [35S]l-methionine and [35S]l-cysteine for 15 min at 37°C. Cell lysates from equal cell numbers were analyzed on an SDS-PAGE gel. Radiolabeled proteins were visualized using a phosphorlmager. The image is representative of results from three independent experiments.

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  FIG 4. Effects of neomycin on CVB3-Nancy replication kinetics. Shown is the growth curve in the presence of liquid media (A) or agar overlay (B). Briefly, 1 × 106 HeLa cells that had been pretreated or not with neomycin were inoculated with CVB3-Nancy at an MOI of 0.01. Virus was incubated for 30 min at 37°C, and either DMEM liquid (A) or 1% agar–1% DMEM mixture (B) with or without neomycin was added. At the indicated time points, intracellular virus was harvested and quantified by plaque assay. For panels A and B, n = 4 or 5 total data points from two independent experiments. To examine multicycle replication and spread, cells were infected with 100 PFU of CVB3-Nancy and either DMEM (C) or 1% agar–1% DMEM mixture (D) with or without neomycin, followed by plaque assay of cell-associated virus. For panels C and D, n = 6 total data points from three independent experiments. (E) HeLa cells were pretreated or not with 1 mg/ml of neomycin prior to plating of 100 PFU of CVB3-Nancy on cells with agar overlays with or without 1 mg/ml of neomycin. Plates were stained with crystal violet 48 hpi. *, P < 0.05 (unpaired two-tailed Student t tests). Images are representative of results from two…

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  FIG 5. Effect of positively or negatively charged compounds on CVB3-Nancy and reovirus plaque formation. (A) A total of 8.8 × 106 HeLa cells were infected with 100 PFU of CVB3-Nancy and agar overlays were added with or without 0.1 µM poly-l-lysine or 0.8 mg/ml of protamine (positively charged compounds). (B) A total of 1 × 106 L929 cells were infected with 100 PFU of type 3 Dearing reovirus and agar overlays were added with or without 0.8 mg/ml of protamine. (C) A total of 8.8 × 106 HeLa cells were infected with 100 PFU of CVB3-Nancy and neomycin-containing agar or agarose overlays were added with or without 1 mg/ml of heparin (negatively charged compound). Images are representative of results from two independent experiments.

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  FIG 6. Effects of positively charged compounds on CVB3-Nancy diffusion. A total of 1 × 106 HeLa cells in 60-mm tissue culture plates were overlaid with 4 ml of 1% agar–1% DMEM mixture that contained or lacked 1 mg/ml of neomycin or 0.8 mg/ml of protamine. Once the overlay solidified, 5 × 104 PFU of CVB3-Nancy in 200 µl was added dropwise to the top of the overlay. Cells were placed at 37°C to allow diffusion of the virus through the overlay to the cell monolayer, and plates were stained with crystal violet at 1, 2, or 3 days postinfection (dpi) to reveal the extent of cell death from viral replication. Images are representative of results from two independent experiments.