Key result
Heparin and bFGF loaded onto decellularized porcine aortic heart valve leaflets via layer-by-layer deposition showed sustained release over 4 days while preserving biological activity.
Layer-by-layer electrostatic deposition is a viable technique for loading and sustaining the release of biologically active growth factors from decellularized aortic valve scaffolds.
Aortic heart valve disease is a growing health problem and a tissue-engineered aortic heart valve could be a promising therapy. In this paper, decellularized porcine aortic heart valve leaflets are used as scaffolds and loaded with growth factor and heparin via layer-by-layer electrostatic deposition (LbL technique) with the final purpose to stimulate and control cellular processes. Binding and subsequent release of heparin and basic fibroblast growth factor (bFGF) from aortic valve leaflets were assessed qualitatively by immunohistochemistry and quantitatively by radioactive labeling methods. It was observed that the amount of heparin and bFGF bound to aortic heart valve leaflets was directly proportional to the concentration of heparin and bFGF in the incubation medium. Release of heparin and bFGF from the decellularized heart valve leaflets at physiological conditions was sustained over 4 days while preserving the biological activity of the released growth factor.
No takes yet. Share an insight, caveat, or question.
Cock et al. (2010) studied Aortic heart valve disease. Layer-by-layer electrostatic deposition of heparin and basic fibroblast growth factor (bFGF) was evaluated on Binding and subsequent release of heparin and bFGF. Heparin and bFGF loaded onto decellularized porcine aortic heart valve leaflets via layer-by-layer deposition showed sustained release over 4 days while preserving biological activity.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: