Key result
Electrospun hybrid heart valve scaffolds improve recellularization, cell mass, and mechanical strength versus controls.
Why the study?
Does electrospinning with basic fibroblast growth factor/chitosan/poly-4-hydroxybutyrate improve recellularization and mechanical strength in decellularized porcine aortic heart valve leaflets seeded with rat mesenchymal stem cells?
Does electrospinning with basic fibroblast growth factor/chitosan/poly-4-hydroxybutyrate improve recellularization and mechanical strength in decellularized porcine aortic heart valve leaflets seeded with rat mesenchymal stem cells?
A novel electrospinning technique using basic fibroblast growth factor/chitosan/poly-4-hydroxybutyrate significantly improved recellularization and mechanical strength of tissue-engineered heart valve leaflets in vitro.
The objective of this study was to fabricate biomatrix/polymer hybrid heart valve leaflet scaffolds using an electrospinning technique and seeded by mesenchymal stem cells. Mesenchymal stem cells were obtained from rats. Porcine aortic heart valve leaflets were decellularized, coated with basic fibroblast growth factor/chitosan/poly-4-hydroxybutyrate using an electrospinning technique, reseeded, and cultured over a time period of 14 days. Controls were reseeded and cultured over an equivalent time period. Specimens were examined biochemically, histologically, and mechanically. Recellularization of the hybrid heart valve leaflet scaffolds was significantly improved compared to controls. Biochemical and mechanical analysis revealed a significant increase of cell mass, 4-hydroxyproline, collagen, and strength in the hybrid heart valve leaflets compared to controls. This is the first attempt in tissue-engineered heart valves to fabricate hybrid heart valve leaflets using mesenchymal stem cells combined with a slow release technique and an electrospinning technique.
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Hong et al. (2009) studied Tissue-engineered heart valves. Biomatrix/polymer hybrid heart valve leaflet scaffolds using electrospinning and mesenchymal stem cells vs. Controls (reseeded and cultured over an equivalent time period) was evaluated on Recellularization, cell mass, 4-hydroxyproline, collagen, and strength. Hybrid heart valve leaflet scaffolds fabricated using electrospinning and seeded with mesenchymal stem cells significantly improved recellularization, cell mass, and strength compared to controls.
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