Key result
PhAETP, unlike NPhAETP or ATP, does not form a hydrogen bond with Tyr-134 in myosin subfragment 1, providing a structural reason for its inability to support active tension development.
Population
Myosin subfragment 1 (S1)
Comparison
2-[amino]ethyl triphosphate and 2-ethyl… vs ATP
Design
Preclinical
Authors
Loading...
Distinguishes myosin-nucleotide specificity in tension generation; leaves open translation to human muscle physiology or therapies.
The lack of a hydrogen bond with Tyr-134 in S1 explains why PhAETP, unlike NPhAETP or ATP, fails to support active tension development in muscle fibers.
Moschcovich et al. (1998) studied this question. NPhAETP and PhAETP vs. ATP was evaluated on S1-catalyzed hydrolysis, formation of stable complexes, and effect on S1 conformation. PhAETP, unlike NPhAETP or ATP, does not form a hydrogen bond with Tyr-134 in myosin subfragment 1, providing a structural reason for its inability to support active tension development.