Based on 4-(1-pyrenyl)-1, 3-butanediol, a pyrene seco-pseudonucleoside, we prepared a nonnucleotide phosphoramidite reagent and a pyrene-modified LCAA-CPG-support and employed them in solid-phase synthesis of pyrene-labeled oligonucleotides with varying numbers and positions of the pseudonucleoside residues. Changes in the fluorescence intensity and the spectral curve shape upon hybridization were observed that depended on the position(s) of the modified unit(s) in the probe.
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Korshun et al. (1999) studied this question.
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