Key result
An indirect solid-phase enzyme immunoassay was developed to detect a minimum of 2.3 µg/ml poliovirus antigen with a coefficient of variation between 2.1% to 7.8%.
The developed indirect solid-phase EIA can reliably detect poliovirus antigen with a minimum detection limit of 2.3 µg/ml.
May aid poliovirus antigen detection in preclinical models; leaves open human diagnostic validation.
An indirect solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Virus antigen was obtained in LLC-MK2 cell cultures and used to prepare antibodies in rabbit and guinea pig. Antibodies were evaluated by double immunodiffusion and neutralization test. Optimal concentrations of guinea pig and rabbit immunoglobulins were determined by checkerboard titration. Microtitre plates were coated with 15.0 µg/ml guinea pig anti-polio immunoglobulin and rabbit anti-polio immunoglobulin at the concentration of 7.94 µg/ml was used as detecting antibody. The standard curve with eight different antigen concentrations in eight replicates resulted in a coefficient of variation (CV) between 2.1% to 7.8%. The dose-response relationship was determined by simple linear regression with a coefficient of correlation (R²) equal to 96.4%. The assay detected a minimum of 2.3 µg/ml poliovirus antigen.
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Hashimoto et al. (2007) studied Poliovirus antigen detection. Indirect solid-phase enzyme immunoassay (EIA) was evaluated on Detection of poliovirus antigen. An indirect solid-phase enzyme immunoassay was developed to detect a minimum of 2.3 µg/ml poliovirus antigen with a coefficient of variation between 2.1% to 7.8%.
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