Key result
Antisera raised against polioviral proteins VP0, VP3, VP1, and 3AB expressed in E. coli successfully detected all three serotypes of poliovirus in ELISA, immunofluorescence, and western blotting.
The study successfully generated non-infectious polioviral protein reagents and antisera that can detect all three poliovirus serotypes, which may be useful for diagnostic tests during the final phase of polio eradication.
Antisera enable poliovirus detection across assays; leaves open validation for human diagnostics in eradication programs.
Poliomyelitis, caused by poliovirus, is on the verge of eradication, and the world is preparing to shift from live to inactivated polio vaccine. In view of the requirement of non-infectious reagents, especially protein antigens, for surveillance during the final phase of poliovirus eradication, we have attempted to generate reagents that may be of use for the development of diagnostic tests. Polioviral proteins VP0, VP3, VP1, and 3AB were expressed in Escherichia coli using the autoinduction system, purified, and the proteins were used to raise antisera in rabbits. All antisera detected all three serotypes of PV from infected cell lysates in enzyme-linked immunosorbent assay, immunofluorescence and western blotting.
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Uma et al. (2016) studied Poliomyelitis. Polioviral proteins VP0, VP3, VP1, and 3AB was evaluated on Detection of poliovirus serotypes in immunological assays. Antisera raised against polioviral proteins VP0, VP3, VP1, and 3AB expressed in E. coli successfully detected all three serotypes of poliovirus in ELISA, immunofluorescence, and western blotting.
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