, extending earlier work by Pressman (1943) defined some of the variables influencing colour yield. Herriott (1941) discovered that pretreatment with cupric ion greatly intensified the blue colour given by proteins and peptides, but had no effect upon the colour .yield from tyrosine. Lowry, Rose- brough, Farr & Randall (1951) further refined and systematized these observations into a practical method of protein assay which, because of its
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Chou et al. (1960) studied this question.
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