Key result
Spectroscopic data suggest ouabain binds at two sites (an external low-affinity site and a deeper high-affinity site) along the ion permeation pathway of the Na+/K+ ATPase.
Population
Xenopus laevis oocytes expressing five normally functional LBT-Na(+)/K(+) ATPase constructs
Design
Preclinical
Authors
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No immediate clinical implications from this animal study; leaves open functional validation of dual-site ouabain binding in vivo.
The study proposes a sequential ouabain binding mechanism to the Na+/K+ ATPase involving two distinct binding sites along the ion permeation pathway.
Sandtner et al. (2011) studied this question. Fluorescent ouabain and lanthanide binding tag (LBT) was evaluated on Distance between LBT and fluorescent ouabain / ouabain binding sites. Spectroscopic data suggest ouabain binds at two sites (an external low-affinity site and a deeper high-affinity site) along the ion permeation pathway of the Na+/K+ ATPase.