What is the clinical evidence from this study?

Study design: Other. Intervention: Random mutagenesis of the Na+/K+-ATPase alpha1 subunit. Primary outcome: Identification of amino acid residues altering ouabain sensitivity.

September 1, 1997European Journal of BiochemistryOpen Access

Extensive Random Mutagenesis Analysis of the Na+/K+‐ATPase α Subunit Identifies Known and Previously Unidentified Amino Acid Residues that Alter Ouabain Sensitivity Implications for Ouabain Binding

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Key result

Random mutagenesis of the Na+/K+-ATPase alpha1 subunit identified 24 ouabain-resistant clones, revealing new substitutions in the H4 and H10 transmembrane regions that alter ouabain sensitivity.

Population

HeLa cells transfected with random mutant libraries of the sheep Na+/K+-ATPase alpha1 subunit

Design

Preclinical

Authors

MCMichelle CroyleWashington University in St. Louis AWAlison L. WooUniversity of Cincinnati Medical Center JLJerry B. LingrelUniversity of Cincinnati Medical Center

Discussion

Member takes

Implication

No immediate clinical implications; extends mutagenesis mapping of Na+/K+-ATPase glycoside sites.

Structured PICO

Population

HeLa cells transfected with random mutant libraries of the sheep Na+/K+-ATPase alpha1 subunit

Intervention

Random mutagenesis and ouabain selection

Outcome

Identification of amino acid residues that alter ouabain sensitivity

Extensive random mutagenesis of the Na+/K+-ATPase alpha1 subunit identified novel amino acid residues in the H4 and H10 transmembrane regions that alter sensitivity to cardiac glycosides like ouabain.

Cite This Study

Croyle et al. (1997) studied this question. Random mutagenesis of the Na+/K+-ATPase alpha1 subunit was evaluated on Identification of amino acid residues altering ouabain sensitivity. Random mutagenesis of the Na+/K+-ATPase alpha1 subunit identified 24 ouabain-resistant clones, revealing new substitutions in the H4 and H10 transmembrane regions that alter ouabain sensitivity.

synapsesocial.com/papers/6aa033f41b0c721c49824994 https://doi.org/10.1111/j.1432-1033.1997.00488.x

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Extensive Random Mutagenesis Analysis of the Na+/K+‐ATPase α Subunit Identifies Known and Previously Unidentified Amino Acid Residues that Alter Ouabain Sensitivity Implications for Ouabain Binding

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Extensive Random Mutagenesis Analysis of the Na+/K+‐ATPase α Subunit Identifies Known and Previously Unidentified Amino Acid Residues that Alter Ouabain Sensitivity Implications for Ouabain Binding

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

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