What question did this study set out to answer?

Determine the spatial distribution of DNA lesions induced by benzo(a)pyrene and its active metabolite BPDE across chromatin structural domains in mammalian cells.

January 1, 1981Nucleic Acids ResearchOpen Access

The distribution of benzo(a)pyrene DNA adducts in mammalian chromatin

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Authors

PJPeter JackRAGT Seeds (United Kingdom)PBP. BrookesThe Wistar Institute

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Implication

Laboratory investigation reveals preferential binding of benzo(a)pyrene to linker DNA over nucleosome cores in mammalian cells, suggesting time-dependent loss of lesion localization.

Key Points

Determine the spatial distribution of DNA lesions induced by benzo(a)pyrene and its active metabolite BPDE across chromatin structural domains in mammalian cells.
Treated murine erythroleukaemic cells and primary mouse embryo cells with either benzo(a)pyrene (BP) or its active metabolite BPDE.
Assayed lesion localization in isolated nuclei using micrococcal nuclease digestion followed by trichloroacetic acid precipitation, hydroxyapatite chromatography, and gel electrophoresis.
Micrococcal nuclease digestion immediately following BPDE exposure demonstrated approximately threefold higher adduct binding in linker DNA regions compared to nucleosome core DNA.
Prolonged incubation with BP or delayed sampling after BPDE exposure resulted in a loss of preferential linker localization over time.

Cite This Study

Jack et al. (1981) studied this question.

synapsesocial.com/papers/6a9377d123705e96f83239fb https://doi.org/10.1093/nar/9.21.5533

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Implication

Laboratory investigation reveals preferential binding of benzo(a)pyrene to linker DNA over nucleosome cores in mammalian cells, suggesting time-dependent loss of lesion localization.

The distribution of benzo(a)pyrene DNA adducts in mammalian chromatin

Discussion

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Implication

Key Points

Cite This Study

The distribution of benzo(a)pyrene DNA adducts in mammalian chromatin

Discussion

Member takes

Implication

Key Points

Cite This Study

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