Key result
A cleanup procedure using PRP-1 polystyrene cartridges and HPLC successfully purified 125I-labeled atrial natriuretic factor and arginine-vasopressin for radioimmunoassays.
A simple and rapid cleanup procedure using PRP-1 polystyrene cartridges and HPLC provides high specific activity for radioiodinated peptides like atrial natriuretic factor.
A simple and rapid cleanup procedure is described for the purification of iodinated peptides using PRP-1 polystyrene cartridges following the radioiodination process. The method is validated using different volumes and solvent systems and compared to the standard Sep-Pak C18 procedure. In this study, the method is used to prepare 125I-labeled atrial natriuretic factor and arginine-vasopressin which are further purified by reverse phase HPLC giving maximally obtainable specific activity required for the radioimmunoassays of these peptides.
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Ong et al. (1987) studied this question. PRP-1 polystyrene cartridges and HPLC vs. Sep-Pak C18 procedure was evaluated on Purification of iodinated peptides. A cleanup procedure using PRP-1 polystyrene cartridges and HPLC successfully purified 125I-labeled atrial natriuretic factor and arginine-vasopressin for radioimmunoassays.
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