This preclinical study successfully purified three biologically active rat atrial natriuretic factors, identifying the 26-amino acid peptide as the most active.
Offers tools for natriuretic peptide research; leaves open therapeutic translation in human cardiovascular disease.
A natriuretic factor has been described in the specific granules of rat atria. We have purified three factors which seem to be low-Mr peptides. They have been purified by means of acid extraction, octadecyl Sep-Pak cartridges, and chromatography on Bio-Gel P-10, CM Bio-Gel A, Mono S and reverse-phase high-performance liquid chromatography columns. The factors contain 26, 31 and 33 amino acids and may have been partially degraded during isolation. They are all 3 biologically active and the shorter one is the most active with a specific activity of 450 000 units/mg.
No takes yet. Share an insight, caveat, or question.
Thibault et al. (1983) studied this question.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: