May 1, 1986Experimental Biology and Medicine

Identification of the Released form of Atrial Natriuretic Factor by the Perfused Rat Heart

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Population

Isolated perfused rat heart

Design

Preclinical

Authors

GTGaétan ThibaultHeart Failure / Cardiomyopathy RGR. GarcíaUniversidad Complutense de Madrid JGJolanta GutkowskaHeart Failure / Cardiomyopathy

Discussion

Member takes

Implication

Preclinical rat data clarify ANF maturation via tryptic-like cleavage; leaves open human relevance and clinical translation.

Structured PICO

Population

Isolated perfused rat heart

Intervention

Extraction and isolation of Atrial Natriuretic Factor (ANF) from perfusates using C18 Sep-Pak cartridges, immunoaffinity chromatography, and reverse phase HPLC, followed by amino acid sequencing

Outcome

Primary structure of the released Atrial Natriuretic Factor (ANF)

The identification of released ANF as a short peptide (Ser 99-Tyr 126) suggests that its maturation process occurs either intracellularly or during secretion via tryptic-like cleavage.

Cite This Study

Thibault et al. (1986) studied this question.

synapsesocial.com/papers/6a7a28c302ab7a73dd02c0e6 https://doi.org/10.3181/00379727-182-1-rc3

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Identification of the Released form of Atrial Natriuretic Factor by the Perfused Rat Heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Identification of the Released form of Atrial Natriuretic Factor by the Perfused Rat Heart

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider