Key Points
- To develop and evaluate a solid-phase radioimmunoassay for measuring atrial natriuretic factor concentrations in rat atrial tissue homogenates.
- Developed a solid-phase radioimmunoassay utilizing a synthetic 26-amino-acid fragment (8-33 ANF) and rabbit anti-8-33 ANF antiserum.
- Coated assay wells with the synthetic peptide and quantified bound complexes using iodine-125-labeled goat anti-rabbit IgG.
- Measured and compared immunoreactive peptide concentrations across homogenates of rat right and left atria.
- Measured bound radioactivity demonstrated a direct proportionality to the quantity of atrial natriuretic factor present.
- Immunoreactive atrial natriuretic factor concentrations were approximately 4 times higher in the right atrium than in the left atrium.
Structured PICO
PPopulationRat atria homogenates
IInterventionSolid phase radioimmunoassay for atrial natriuretic factor (ANF) using a synthetic 26 amino-acid fragment (8-33 ANF)
OOutcomeConcentration of immunoreactive ANFsurrogate
A newly described solid phase radioimmunoassay successfully measures ANF in rat atria, revealing significantly higher concentrations in the right atrium.