Key result
In the Staphylococcus epidermidis type III CRISPR-Cas system, Csm3 acts as a ruler that binds crRNA with a 6-nucleotide periodicity to determine the extent of its maturation.
Population
Staphylococcus epidermidis type III CRISPR-Cas system (in vitro and in vivo models)
Comparison
Mutagenesis of Csm3 and modulation of Csm3 levels vs Wild-type Csm3
Design
Preclinical
Authors
Loading...
No immediate clinical implications for cardiovascular gene editing; leaves open extension of CRISPR maturation mechanisms to therapeutic contexts.
Csm3 acts as a ruler protein within the Cas10·Csm complex, determining the length of mature crRNAs in 6-nucleotide increments.
Hatoum-Aslan et al. (2013) studied this question. Csm3 mutation and level modulation vs. Wild-type Csm3 was evaluated on crRNA size pattern and maturation. In the Staphylococcus epidermidis type III CRISPR-Cas system, Csm3 acts as a ruler that binds crRNA with a 6-nucleotide periodicity to determine the extent of its maturation.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: