Increased glycolytic capacity and associated enzyme changes in BHK21 cells transformed with polyoma virus

Some features of the metabolism of untransformed and polyoma‐transformed clones of the BHK21 cell strain were studied. 1) No difference in the oxygen uptake of the two groups was detected but in all cases the transformed clones had greater glycolytic capacity than the untransformed clones at pH 7.4, in both aerobic and anaerobic conditions. 2) The transformed clones had more hexokinase and isocitric dehydrogenase, and probably more malic dehydrogenase, than the untransformed clones. 3) The untransformed clones had more glucose‐6‐phosphate dehydrogenase than the transformed clones. 4) No differences could be detected in 6‐phosphogluconate dehydrogenase or lactic dehydrogenase activity. 5) It is suggested that these differences may be related either to differences in the permeability of the cell membranes to glucose or to an altered rate of synthesis of mucopolysaccharides.