Key result
Myosin VIIA interacts with the type I alpha regulatory subunit of protein kinase A, suggesting it acts as a novel A-kinase-anchoring protein in sensory cells of the cochlea and retina.
Myosin VIIA acts as a novel A-kinase-anchoring protein, targeting protein kinase A to specific subcellular sites in sensory cells, which may relate to hereditary hearing loss and visual deficiency.
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May link myosin VIIA-PKA anchoring to sensory cell function; hypothesis-generating for mechanisms of hereditary hearing and vision loss.
To gain an insight into the cellular function of the unconventional myosin VIIA, we sought proteins interacting with its tail region, using the yeast two-hybrid system. Here we report on one of the five candidate interactors we identified, namely the type I alpha regulatory subunit (RI alpha) of protein kinase A. The interaction of RI alpha with myosin VIIA tail was demonstrated by coimmunoprecipitation from transfected HEK293 cells. Analysis of deleted constructs in the yeast two-hybrid system showed that the interaction of myosin VIIA with RI alpha involves the dimerization domain of RI alpha. In vitro binding assays identified the C-terminal "4.1, ezrin, radixin, moesin" (FERM)-like domain of myosin VIIA as the interacting domain. In humans and mice, mutations in the myosin VIIA gene underlie hereditary hearing loss, which may or may not be associated with visual deficiency. Immunohistofluorescence revealed that myosin VIIA and RI alpha are coexpressed in the outer hair cells of the cochlea and rod photoreceptor cells of the retina. Our results strongly suggest that myosin VIIA is a novel protein kinase A-anchoring protein that targets protein kinase A to definite subcellular sites of these sensory cells.
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Küssel‐Andermann et al. (2000) studied Hereditary hearing loss. Myosin VIIA was evaluated on Protein-protein interaction. Myosin VIIA interacts with the type I alpha regulatory subunit of protein kinase A, suggesting it acts as a novel A-kinase-anchoring protein in sensory cells of the cochlea and retina.
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