Key result
NADH oxidation through the respiratory chain counteracted the oxidative decomposition of membrane lipids and inactivation of oxidase activities initiated by adriamycin-Fe3+ complexes in pig heart submitochondrial particles.
NADH oxidation and ADP protect mitochondrial membranes and respiratory chain activity from adriamycin-induced oxidative damage, highlighting potential mechanisms of cardiotoxicity protection.
Answers are grounded in this paper and cite their sources.
Animal data on NADH protection from adriamycin damage should not change practice; leaves open translation to human cardioprotection.
Oxidative decomposition of polyunsaturated fatty acid moieties of membrane lipid in pig heart submitochondrial particles, as initiated by ferric ion complexes of the antineoplastic drug adriamycin and concomitant inactivation of oxidase activities, is counteracted by EDTA, low oxygen pressure, a phenolic antioxidant and NADH oxidation through the respiratory chain but not by scavengers of reactive oxygen species. Protection by NADH is strengthened by removal of cytochrome c from the submitochondrial particles and by antimycin A but abolished by rotenone. Inhibition of cytochrome c oxidase activity by the adriamycin-Fe3+ complex is reversible and activity is recovered upon cholate solubilization of the particles. ADP inhibits binding of the complex to the submitochondrial particles and protects both cytochrome c oxidase activity and membrane lipid. The results are discussed in relation to the possible role of mitochondrial function in protection against free-radical-mediated effects of adriamycin.
No takes yet. Share an insight, caveat, or question.
Erland J.F. Demant (1983) studied this question. NADH oxidation was evaluated on Oxidative decomposition of membrane lipid and inactivation of oxidase activities. NADH oxidation through the respiratory chain counteracted the oxidative decomposition of membrane lipids and inactivation of oxidase activities initiated by adriamycin-Fe3+ complexes in pig heart submitochondrial particles.
Synapse has enriched 10 related papers. Consider them for comparative context: