Different Aspects of Cytochalasin B Blocked Micronucleus Cytome (Cbmn Cyt) Assay as a Comprehensive Measurement Tool for Radiobiological Studies, Biological Dosimetry and Genome Instability

It is now universally accepted that DNA is the main target for damages caused by physical and chemical genotoxicants. Although there are different methods to measure directly the induced DNA damages but due to fast repair processes in cellular environment, most of the damages would be repaired even before sampling, therefore processed DNA damages, i.e. damages le� unrepaired aer ac ng repair machinery is preferable to measure in various exposure scenarios. Various cytogene c end points are introduced and implemented such as metaphase analysis, sister chroma d exchanges, premature chromosome condensa on, transloca on assay and micronucleus assay. All of these methods were extensively used for various purposes but among them micronucleus (MN) assay was found more prac cal because of ease of scoring, poten al for automa on as well as being nearly as sensi ve as other procedures. These characteris cs made MN assay very popular for screening of the effects of various genotoxic agents in vitro and in vivo. In this review we try to summarize the main aspects of applica on of this method in radiobiological studies and genome instability related diseases.