Key result
Rat ventricular muscle is a favorable preparation for voltage-clamp experiments using a double sucrose gap technique, with membrane potential deviations never larger than 15 mV during fast inward current.
Rat ventricular muscle is a favorable preparation for voltage-clamp experiments due to its geometry, which allows for acceptable voltage control.
May aid voltage-clamp studies in cardiac muscle; leaves open translation to human electrophysiology.
In rat ventricular muscle, measurements of the membrane potential with microelectrodes during depolarizing voltage steps showed that deviation of the membrane potential from the command signal were never larger than 15 mV during flow of the fast inward current and that voltage control was regained within 15 ms after the beginning of the voltage step. During the flow of the slow inward current, tail currents elicited by interrupting the time course of the slow current at different time intervals returned exponentially to the steady-state level, thus indicating acceptable voltage control. It is concluded that rat ventricular muscle is a rather favorable preparation for voltage-clapm experiments and this is attributed mainly to the geometry of the preparation.
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Schanne et al. (1979) studied this question. Double sucrose gap technique was evaluated on Voltage control during inward current flow. Rat ventricular muscle is a favorable preparation for voltage-clamp experiments using a double sucrose gap technique, with membrane potential deviations never larger than 15 mV during fast inward current.
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