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May 1, 1979Proceedings of the National Academy of SciencesOpen Access

Structural studies on oncornavirus-related sequences in chicken genomic DNA: two-step analyses of EcoRI and Bgl I restriction digests and tentative mapping of a ubiquitous endogenous provirus digests and tentative mapping of a ubiquitous endogenous provirus.

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Key result

Restriction endonuclease digestion and chromatography of chicken genomic DNA identified a simple pattern common to all individuals studied, allowing for the tentative mapping of a ubiquitous endogenous provirus.

Population

DNA from a variety of uninfected chicken cell types

Design

Preclinical

Authors

WMW.L. McClementsUnited States Military AcademyHHHidesaburô HanafusaUniversité Claude Bernard Lyon 1STS M TilghmanHoward Hughes Medical Institute

Discussion

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Implication

Maps avian endogenous provirus and confirms sarc non-adjacency; leaves open any extension to human cardiovascular retroviral models.

Structured PICO

P
Population
Genomic DNA from uninfected chicken cell types analyzed to map endogenous retrovirus sequences.
I
Intervention
Restriction endonuclease digestion (EcoRI and Bgl I) and RPC-5 ion-exchange chromatography followed by agarose gel electrophoresis
O
Outcome
Detection and mapping of endogenous retrovirus sequences and cellular sequences (sarc) homologous to the viral transforming gene (src)

The study maps endogenous provirus sequences in chicken DNA and confirms that cellular sarc sequences are not adjacent to the endogenous provirus.

Limitations

  • The diagram shows the provirus as a continuous segment but interruption by cellular sequences cannot be excluded.
  • The limited survey did not allow deduction of a simple correlation between a given restriction pattern and phenotype.

Cite This Study

McClements et al. (1979) studied Uninfected chicken cell types (endogenous provirus mapping) (n=19). Restriction endonuclease digestion (EcoRI and Bgl I) and RPC-5 chromatography was evaluated on Restriction map of endogenous provirus sequences. Restriction endonuclease digestion and chromatography of chicken genomic DNA identified a simple pattern common to all individuals studied, allowing for the tentative mapping of a ubiquitous endogenous provirus.

synapsesocial.com/papers/6a93aa72b8961b8efcafdb7ahttps://doi.org/10.1073/pnas.76.5.2165
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Recombination between endogenous and exogenous RNA tumor virus genes as analyzed by nucleic acid hybridization1975 · 52 citations
  2. 2A new method for sequencing DNA.1977 · 8,033 citations
  3. 3RAPID TRANSFORMATION OF CELLS BY ROUS SARCOMA VIRUS1969 · 203 citations
  4. 4Homology between avian oncornavirus RNAs and DNA from several avian species1975 · 47 citations