Key result
The FA strain of mouse encephalomyelitis virus was successfully propagated in suspended cell cultures of mouse embryonic brain and whole mouse embryo, surviving for up to 70 days through serial passages.
The study demonstrates the successful serial propagation of the FA strain of mouse encephalomyelitis virus in suspended cell cultures of mouse embryonic tissues.
It has been reported that various neurotropi.c viruses such as poliomyelitis virus (1-3), rabies virus(4), GDVII strain of mouse encephalomyelitis virus (5-6), Columbia MM virus (7), Columbia SK virus (8) and Venezuelan equine encephalomyelitis virus (9) have been successfully propagated in tissue culture, however, any systemic study concerning the propagation of the FA strain of mouse encephalomyelitis virus in tissuc culture has not yet been reported. The authors have attempted the propagation of this virus in tissue culture and the results obtained are presented as follows.
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Nakagawa et al. (1956) studied Mouse encephalomyelitis virus (FA strain) propagation. Suspended cell culture of mouse embryonic brain and whole mouse embryo vs. Other tissue cultures (human, chick, other mouse tissues) was evaluated on Viral propagation (infectivity titer expressed as 50% infectivity endpoint LD50). The FA strain of mouse encephalomyelitis virus was successfully propagated in suspended cell cultures of mouse embryonic brain and whole mouse embryo, surviving for up to 70 days through serial passages.
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