What question did this study set out to answer?

To determine whether stress-signaling cascades activated by ultraviolet-A irradiation in cell culture arise from extracellular hydrogen peroxide formed via medium components.

January 1, 2003Free Radical Research

Irradiation of Cells with Ultraviolet-A (320-400 nm) in the Presence of Cell Culture Medium Elicits Biological Effects Due to Extracellular Generation of Hydrogen Peroxide

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Authors

AMAndré MahnsBeiersdorf (Germany)IMIra MelchheierHeinrich Heine University Düsseldorf CSChristoph V. SuschekDüsseldorf University Hospital

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Implication

In vitro study shows hydrogen peroxide generation during ultraviolet-A exposure in cell culture media, indicating standard nutrients introduce signaling artifacts.

Key Points

To determine whether stress-signaling cascades activated by ultraviolet-A irradiation in cell culture arise from extracellular hydrogen peroxide formed via medium components.
Irradiated cells with ultraviolet-A light (30 J/cm²) in standard cell culture media versus phosphate-buffered saline.
Quantified hydrogen peroxide concentrations generated in ultraviolet-A-exposed media, including HEPES-buffered media.
Assayed photochemical reactions in isolated solutions containing riboflavin, tryptophan, and HEPES alone or in combination.
Ultraviolet-A irradiation activated ERK 1, ERK 2, and Akt in culture media, whereas cells irradiated in phosphate-buffered saline showed negligible activation.
Exposure of cell culture media to 30 J/cm² ultraviolet-A generated approximately 100 µM hydrogen peroxide, with concentrations at least threefold higher in HEPES-buffered media.
Riboflavin mediated the photooxidation of tryptophan or HEPES, identifying it as the critical driver of extracellular hydrogen peroxide generation.

Cite This Study

Mahns et al. (2003) studied this question.

synapsesocial.com/papers/6a93e2111e998d99dafecc12 https://doi.org/10.1080/1071576031000064702

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Implication

In vitro study shows hydrogen peroxide generation during ultraviolet-A exposure in cell culture media, indicating standard nutrients introduce signaling artifacts.

Irradiation of Cells with Ultraviolet-A (320-400 nm) in the Presence of Cell Culture Medium Elicits Biological Effects Due to Extracellular Generation of Hydrogen Peroxide

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Implication

Key Points

Cite This Study

Irradiation of Cells with Ultraviolet-A (320-400 nm) in the Presence of Cell Culture Medium Elicits Biological Effects Due to Extracellular Generation of Hydrogen Peroxide

Discussion

Member takes

Implication

Key Points

Cite This Study

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