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Roe & Kuether's method (1943) is based upon the red coloration given by the 2:4-dinitrophenylhydrazine derivative of dehydroascorbic acid with 85% H2SO4. This reaction is very sensitive and its specificity, as will be shown, is high. We investigated the method in connexion with another inquiry and record some of our observa- tions.
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Penney et al. (1945) studied this question.
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