What question did this study set out to answer?

To extract and isolate an insulin-like protein from Moringa oleifera leaves and assess its potential hypoglycemic activity.

February 1, 2025ChemistrySelectOpen Access

Isolation and Characterization of an Insulin‐Like Protein From Moringa Oleifera and Estimation of Its Antidiabetic Potential

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Authors

SZSumera ZaibUniversity of Central Punjab IKImtiaz KhanQuaid-i-Azam University LBLaiba BabarUniversity of Central Punjab

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Implication

In vitro study reveals strong carbohydrate enzyme inhibition by Moringa oleifera leaf protein, suggesting therapeutic utility for diabetes management.

Key Points

To extract and isolate an insulin-like protein from Moringa oleifera leaves and assess its potential hypoglycemic activity.
Extracted leaf proteins using a Tris-HCl buffer solution, followed by filtration, centrifugation, and dialysis to obtain purified supernatant and pellet fractions.
Measured total protein concentration using a serum albumin assay and confirmed the presence of an insulin-like protein via zinc chloride (ZnCl2) precipitation.
Evaluated antidiabetic potential using in vitro alpha-amylase and alpha-glucosidase enzyme inhibition assays.
Total protein content was 8.0 ± 0.25 g/dL in both the supernatant and pellet fractions.
The extract demonstrated strong alpha-amylase inhibition of 75.48% (IC50 = 0.6 µM) for the supernatant and 82.93% (IC50 = 17.6 µM) for the pellet.
Alpha-glucosidase inhibition was modest, reaching 11.1% in the supernatant and 14.1% in the pellet fraction.

Cite This Study

Zaib et al. (2025) studied this question.

synapsesocial.com/papers/6a93f7f27fb89795fbfece03 https://doi.org/10.1002/slct.202401991

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Implication

In vitro study reveals strong carbohydrate enzyme inhibition by Moringa oleifera leaf protein, suggesting therapeutic utility for diabetes management.

Isolation and Characterization of an Insulin‐Like Protein From Moringa Oleifera and Estimation of Its Antidiabetic Potential

Discussion

Member takes

Implication

Key Points

Cite This Study

Isolation and Characterization of an Insulin‐Like Protein From Moringa Oleifera and Estimation of Its Antidiabetic Potential

Discussion

Member takes

Implication

Key Points

Cite This Study

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