What question did this study set out to answer?

To examine the quantitative relationship between cyclic AMP formation and lipolysis under normal and acidic conditions, and to evaluate the role of endogenous adenosine in mediating the antilipolytic effects of acidosis.

February 1, 1976Acta Physiologica Scandinavica

Cyclic AMP‐Dependent and Independent Inhibition of Lipolysis by Adenosine and Decreased pH

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Authors

PHPaul HjemdahlPreventive Cardiology BFBertil B. FredholmKarolinska Institutet

Discussion

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Implication

Laboratory study demonstrates that reduced pH and adenosine inhibit fat breakdown in rat adipocytes, indicating dual cyclic AMP-dependent and independent mechanisms.

Key Points

To examine the quantitative relationship between cyclic AMP formation and lipolysis under normal and acidic conditions, and to evaluate the role of endogenous adenosine in mediating the antilipolytic effects of acidosis.
Isolated rat adipocytes were incubated at normal (pH 7.4) and reduced pH (pH 6.6) conditions.
Cells were treated with noradrenaline, adenosine, adenosine deaminase (ADA), or theophylline to assess cyclic AMP accumulation and glycerol release.
Acidosis potentiated the inhibitory effects of adenosine on cyclic AMP accumulation and lipolysis, while adenosine antagonists (theophylline and ADA) stimulated both processes.
Decreasing pH from 7.4 to 6.6 increased the cyclic AMP required for half-maximal lipolysis activation from 1.3 to 3.1 pMol × 10⁻⁵ cells and reduced maximal glycerol production from 1,300 to 900 nMol × 10⁻⁵ cells.
Acidosis inhibited cyclic AMP accumulation and lipolytic responses induced by theophylline and ADA to a greater extent than those stimulated by noradrenaline.

Cite This Study

Hjemdahl et al. (1976) studied this question.

synapsesocial.com/papers/6a9402f38e5b8037ce43f232 https://doi.org/10.1111/j.1748-1716.1976.tb10186.x

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Implication

Laboratory study demonstrates that reduced pH and adenosine inhibit fat breakdown in rat adipocytes, indicating dual cyclic AMP-dependent and independent mechanisms.

Cyclic AMP‐Dependent and Independent Inhibition of Lipolysis by Adenosine and Decreased pH

Discussion

Member takes

Implication

Key Points

Cite This Study

Cyclic AMP‐Dependent and Independent Inhibition of Lipolysis by Adenosine and Decreased pH

Discussion

Member takes

Implication

Key Points

Cite This Study

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