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January 1, 1996Japanese Circulation Journal-english EditionOpen Access

Streptozotocin-induced diabetes reduced mitochondrial state 3 respiration to 59.1% of control, and complex I and V activities to 43.4% and 71.7%, all of which recovered with insulin treatment.

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Why the study?

Does insulin treatment improve mitochondrial respiratory impairment in streptozotocin-induced diabetic rat hearts?

Population

Male Wistar rats with diabetes mellitus induced by intravenous injection of streptozotocin (STZ)

Comparison

Insulin injection for 2 or 3 weeks prior to… vs Diabetic rats without insulin treatment and…

Design

Preclinical

Follow-up

2 to 16 weeks

Key result

Streptozotocin-induced diabetes reduced mitochondrial state 3 respiration to 59.1% of control, and complex I and V activities to 43.4% and 71.7%, all of which recovered with insulin treatment.

Authors

MTMasataka TomitaSMSyuji MukaeEGEiichi Geshi

Discussion

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Overview

Insulin may restore mitochondrial function in diabetic rat hearts; hypothesis-generating and should not change practice.

Structured PICO

Does insulin treatment improve mitochondrial respiratory impairment in streptozotocin-induced diabetic rat hearts?

P
Population
Male Wistar rats with streptozotocin-induced diabetes mellitus, with or without insulin treatment, evaluated for 2 to 16 weeks.
I
Intervention
Insulin injection for 2 or 3 weeks prior to sacrifice
C
Comparator
Diabetic rats without insulin treatment and non-diabetic control rats
O
Outcome
Mitochondrial respiratory function (state 3 respiration, complex I and complex V activities)surrogate

In a diabetic rat model, impaired oxidative phosphorylation due to depressed complex I and V activities contributes to cardiac mitochondrial dysfunction, which is reversible with insulin treatment.

Cite This Study

Tomita et al. (1996) studied Diabetes mellitus. Streptozotocin (STZ) vs. Control and insulin-treated diabetic rats was evaluated on Mitochondrial state 3 respiration. Streptozotocin-induced diabetes reduced mitochondrial state 3 respiration to 59.1% of control, and complex I and V activities to 43.4% and 71.7%, all of which recovered with insulin treatment.

synapsesocial.com/papers/6a94069c01d6912b871d4f48https://doi.org/10.1253/jcj.60.673
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