Key result
A cardiac muscle-specific enhancer containing a CArG motif was localized between -136 and -700 bp of the ANF gene, regulating its developmental stage-specific transcription in cardiac cells.
Population
Atrial and ventricular cardiocyte cultures prepared from 18 or 19 embryonic, 0- to 1-, and 4- to 5-day-old…
Comparison
Transfection with ANF promoter constructs linked… vs Non-cardiac cells and control promoters
Design
Preclinical
Authors
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No immediate clinical impact; leaves open translational relevance of ANF enhancers and requires human validation studies.
This in vitro model identifies stage-specific regulatory mechanisms, including a CArG element, for ANF gene transcription during postnatal cardiac differentiation.
Argentin et al. (1994) studied Cardiac gene expression (ANF). ANF promoter deletion and mutation constructs vs. Wild-type or control constructs was evaluated on ANF promoter activity. A cardiac muscle-specific enhancer containing a CArG motif was localized between -136 and -700 bp of the ANF gene, regulating its developmental stage-specific transcription in cardiac cells.
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