Key result
Postfixation of glutaraldehyde-treated pericardium with glycine and gentamicin prevented calcification (0.32 +/- 0.13 mg/g calcium) at 12 weeks of subcutaneous implantation in rats.
Why the study?
Does postfixation with amino compounds prevent calcification of glutaraldehyde-treated pericardium implanted subcutaneously in rats?
Does postfixation with amino compounds prevent calcification of glutaraldehyde-treated pericardium implanted subcutaneously in rats?
Postfixation with glycine and gentamicin effectively prevents calcification of glutaraldehyde-treated pericardium in a rat model, suggesting a potential method to improve bioprosthetic tissue durability.
May mitigate bioprosthetic calcification; hypothesis-generating for human valve durability.
An attempt has been made to compare the role of different amino compounds in prevention of calcification of glutaraldehyde (GA)-treated pericardium implanted subcutaneously in the rat for 12 weeks. GA pretreated pericardial samples were postfixed separately with glycine, albumin, gentamicin, glycine + gentamicin, and albumin + gentamicin. Severe calcification was noticed in animals undergoing albumin or gentamicin postfixation. Mild calcification was observed in glycine and albumin + gentamicin postfixed materials whereas calcium was undetectable either chemically or morphologically in GA-pretreated pericardium postfixed with glycine + gentamicin (0.32 +/- 0.13.mg/g calcium, dry wt; von Kossa 0) at the 12th week of implantation in the rat.
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Jyotirmay Chanda (1994) studied Calcification of glutaraldehyde-treated pericardium. Postfixation with glycine and gentamicin vs. Postfixation with glycine, albumin, gentamicin, or albumin and gentamicin was evaluated on Calcification (calcium level). Postfixation of glutaraldehyde-treated pericardium with glycine and gentamicin prevented calcification (0.32 +/- 0.13 mg/g calcium) at 12 weeks of subcutaneous implantation in rats.
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