What question did this study set out to answer?

To evaluate and compare the transfection efficiency and cellular safety of several commercial gene delivery reagents in NG108-15, SH-SY5Y, and CHO-K1 cell lines.

January 1, 2010Methods and Findings in Experimental and Clinical Pharmacology

Efficiency of gene transfection reagents in NG108-15, SH-SY5Y and CHO-K1 cell lines

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Authors

EMElisa Martín‐MontañezInstituto de Investigación Biomédica de Málaga JLJuan F. López‐TéllezUniversidad de Málaga MAMaria Acevedo-CaladoThe University of Texas MD Anderson Cancer Center

Discussion

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Implication

In vitro study demonstrates variable transfection efficiency across mammalian cell lines, indicating Lipofectamine 2000 is optimal without inducing deformation.

Key Points

To evaluate and compare the transfection efficiency and cellular safety of several commercial gene delivery reagents in NG108-15, SH-SY5Y, and CHO-K1 cell lines.
Cloned regulator of G-protein signaling (RGS) genes up to 2.2 kb in size into a nonviral pcDNA 6.2/EmGFP vector fused with a 3' green fluorescent protein tag.
Transfected NG108-15, SH-SY5Y, and CHO-K1 cell lines using reagents including Lipofectamine 2000, ExGen 500, and TurboFectin 8.0.
Quantified transfection efficiency via flow cytometric measurement of green fluorescent protein expression and assessed cellular morphology.
Lipofectamine 2000 and ExGen 500 achieved 60% to 80% transfection efficiency in NG108-15 cells, and together with TurboFectin 8.0 reached 60% to 80% efficiency in CHO-K1 cells.
TurboFectin 8.0 performed best in SH-SY5Y cells but yielded only a 5% transfection efficiency, with gene size having no discernible effect on delivery rates across all lines.
ExGen 500 caused noticeable morphological deformation in transfected cells, whereas Lipofectamine 2000 preserved normal cell morphology.

Cite This Study

Martín‐Montañez et al. (2010) studied this question.

synapsesocial.com/papers/6a9427e7bbea1c735e92d84d https://doi.org/10.1358/mf.2010.32.5.1498327

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Implication

In vitro study demonstrates variable transfection efficiency across mammalian cell lines, indicating Lipofectamine 2000 is optimal without inducing deformation.

Efficiency of gene transfection reagents in NG108-15, SH-SY5Y and CHO-K1 cell lines

Discussion

Member takes

Implication

Key Points

Cite This Study

Efficiency of gene transfection reagents in NG108-15, SH-SY5Y and CHO-K1 cell lines

Discussion

Member takes

Implication

Key Points

Cite This Study

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