Key result
Rotavirus variants encoding defective NSP1 exhibited a small-plaque phenotype, and an atypical 3' terminal sequence (UGAACC) reduced the efficiency of minus-strand synthesis and gene expression.
Population
Simian SA11 rotaviruses in cell culture
Comparison
Intragenic rearrangement and changes in the 3'… vs Wild-type viruses
Design
Preclinical
Authors
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May attenuate rotavirus in cell culture; leaves open roles in pathogenesis and vaccine attenuation pending in vivo studies.
The 3' consensus sequence of SA11 rotavirus gene 5 dsRNAs has undergone a mutation that operates suboptimally in RNA replication and NSP1 expression, which may provide a selective advantage in cell culture.
Patton et al. (2001) studied Rotavirus infection (in vitro). Intragenic rearrangement and 3' consensus sequence mutation vs. Wild-type rotavirus and consensus sequence was evaluated on NSP1 expression, viral replication efficiency, and minus-strand synthesis. Rotavirus variants encoding defective NSP1 exhibited a small-plaque phenotype, and an atypical 3' terminal sequence (UGAACC) reduced the efficiency of minus-strand synthesis and gene expression.
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