June 1, 2000RNAOpen Access

A four-nucleotide translation enhancer in the 3′-terminal consensus sequence of the nonpolyadenylated mRNAs of rotavirus

View Full Paper

Ask AI

Bookmark

Population

Rotavirus-infected cells and uninfected cells (in vitro model)

Comparison

Chimeric RNAs containing an open reading frame… vs Uninfected cells, or RNAs without the 3'-GACC…

Design

Preclinical

Authors

Discussion

Does not yet alter rotavirus management; leaves open whether GACC targeting yields antiviral strategies.

Population

Rotavirus-infected cells and uninfected cells (in vitro model)

Intervention

Chimeric RNAs containing an open reading frame for luciferase and the 5' and 3' UTRs of a rotavirus mRNA (specifically testing the 3'-GACC element)

Comparator

Uninfected cells, or RNAs without the 3'-GACC element

Outcome

Gene expression (luciferase translation/protein expression)surrogate

Identifies a novel 4-nucleotide 3' translation enhancer (GACC) in rotavirus mRNAs that stimulates translation in infected cells.

Chizhikov et al. (2000) studied this question.

View Full Paper

Ask AI

Bookmark