Cap-independent enhancement of translation by a plant potyvirus 5' nontranslated region
Key result
The 144-nucleotide 5' nontranslated region of the tobacco etch virus genome enhanced translation by 8- to 21-fold in a cap-independent manner compared to a synthetic 5' NTR.
Population
Protoplasts, transgenic plants, and cell-free translation systems
Comparison
144-nucleotide 5' nontranslated region of the… vs Synthetic/artificial 5' NTR fused to GUS
Design
Preclinical
Authors
Discussion
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Implication
Hypothesis-generating for viral vector design; leaves open translation to cardiovascular gene therapy.
Structured PICO
P
Population
Protoplasts, transgenic plants, and cell-free translation systems
I
Intervention
144-nucleotide 5' nontranslated region (NTR) of the tobacco etch virus (TEV) genome fused to a reporter gene encoding beta-glucuronidase (GUS)
C
Comparator
Synthetic/artificial 5' NTR fused to GUS
O
Outcome
Translation enhancement (GUS expression)surrogate
Main Result
Effect estimate: 8- to 21-fold enhancement
The 5' NTR of the tobacco etch virus promotes translation in a cap-independent manner, likely via internal binding of translation factors or ribosomes.
Cite This Study
Carrington et al. (1990) studied Tobacco etch virus (TEV) translation. TEV 144-nucleotide 5' nontranslated region (NTR) vs. Synthetic/artificial 5' NTR was evaluated on Translation enhancement (GUS reporter gene expression) (8- to 21-fold enhancement). The 144-nucleotide 5' nontranslated region of the tobacco etch virus genome enhanced translation by 8- to 21-fold in a cap-independent manner compared to a synthetic 5' NTR.