Key result
Mutations to the TEV 5'-leader pseudoknot 1 disrupted cap-independent translation, indicating the 5'-leader functionally substitutes for a 5'-cap through a 45-nucleotide pseudoknot-containing domain.
Population
Tobacco etch virus (TEV) 5'-leader RNA
Comparison
Mutational analysis of the TEV 5'-leader… vs Wild-type TEV 5'-leader
Design
Preclinical
Authors
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Hypothesis-generating for pseudoknot-driven translation; leaves open mammalian validation before any translational applications.
The TEV 5'-leader functionally substitutes for a 5'-cap and promotes cap-independent translation through a 45-nucleotide pseudoknot-containing domain.
Zeenko et al. (2005) studied this question. Mutational analysis of the TEV 5'-leader was evaluated on Cap-independent translation. Mutations to the TEV 5'-leader pseudoknot 1 disrupted cap-independent translation, indicating the 5'-leader functionally substitutes for a 5'-cap through a 45-nucleotide pseudoknot-containing domain.
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