Key result
eIF4G binds the tobacco etch virus 5'-leader and PK1 RNA with approximately 22-30-fold stronger affinity than eIFiso4G, demonstrating a direct interaction without other initiation factors.
Population
Tobacco etch virus (TEV) 143-nt 5'-leader (TEV1-143), a shorter RNA containing PK1, and a mutant (S1-3)
Design
Preclinical
Authors
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Provides mechanistic insight into potyvirus translation; leaves open whether eIF4G affinity differences affect viral replication in vivo.
Effect estimate: 22-30-fold stronger affinity
This study demonstrates the first direct interaction of eIF4G with the TEV 5'-leader in the absence of other initiation factors, providing insight into the translational strategy of potyviruses.
Ray et al. (2006) studied this question. eIF4G vs. eIFiso4G was evaluated on Binding affinity to TEV1-143 and PK1 RNA (22-30-fold stronger affinity). eIF4G binds the tobacco etch virus 5'-leader and PK1 RNA with approximately 22-30-fold stronger affinity than eIFiso4G, demonstrating a direct interaction without other initiation factors.
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