We report on the perturbations on the retention parameter in hollow-fiber flow field-flow fractionation that is caused by sample overloading. The overloading phenomenon is connected to the sample concentration at the channel wall, and an equation for calculating this concentration is presented. It is shown that the expression can be used to approximate the maximum amount of sample that can be loaded and how it depends on the fiber radius. The loadability dependence on the ionic and field strength is also investigated.
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Carlshaf et al. (1993) studied this question.
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