Key result
The positive-strand RNA secondary structure of the 58-nucleotide internal replication signal, but not the negative-strand structure, is essential for murine coronavirus defective interfering RNA replication.
Population
Mouse DBT cells and mouse L2 cells infected with MHV-A59 helper virus
Comparison
Mutational analysis of the 58-nt internal… vs Wild-type (IRWT) defective interfering (DI) RNA
Design
Preclinical
Authors
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No immediate clinical implications from this murine model; extends basic virology but leaves open human relevance.
The positive-strand RNA secondary structure of the 58-nt internal replication signal is essential for murine coronavirus defective interfering RNA synthesis.
Repass et al. (1998) studied Murine Coronavirus (MHV) replication. Mutations in the 58-nt internal replication signal vs. Wild-type (IRWT) sequence was evaluated on Positive-strand DI RNA synthesis/replication efficiency. The positive-strand RNA secondary structure of the 58-nucleotide internal replication signal, but not the negative-strand structure, is essential for murine coronavirus defective interfering RNA replication.
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