Key result
Mutation of the invariant apical stem of a picornavirus IRES abolished IRES activity, demonstrating that a highly interactive structure constrained by distant interactions is necessary for translation.
The invariant apical stem of picornavirus IRES is critical for maintaining the RNA conformation necessary for IRES-mediated translation through distant interactions involving G:C base pairs.
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May inform IRES-targeted antivirals; leaves open in vivo validation and clinical translation.
RNA structure plays a fundamental role in internal initiation of translation. Picornavirus internal ribosome entry site (IRES) are long, efficient cis-acting elements that recruit the ribosome to internal mRNA sites. However, little is known about long-range constraints determining the IRES RNA structure. Here, we sought to investigate the functional and structural relevance of the invariant apical stem of a picornavirus IRES. Mutation of this apical stem revealed better performance of G:C compared with C:G base pairs, demonstrating that the secondary structure solely is not sufficient for IRES function. In turn, mutations designed to disrupt the stem abolished IRES activity. Lack of tolerance to accept genetic variability in the apical stem was supported by the presence of coupled covariations within the adjacent stem-loops. SHAPE structural analysis, gel mobility-shift and microarrays-based RNA accessibility revealed that the apical stem contributes to maintain IRES RNA structure through the generation of distant interactions between two adjacent stem-loops. Our results demonstrate that a highly interactive structure constrained by distant interactions involving invariant G:C base pairs plays a key role in maintaining the RNA conformation necessary for IRES-mediated translation.
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Fernández et al. (2011) studied this question. Mutation of the apical stem of a picornavirus IRES vs. Wild-type IRES was evaluated on IRES activity and RNA structure maintenance. Mutation of the invariant apical stem of a picornavirus IRES abolished IRES activity, demonstrating that a highly interactive structure constrained by distant interactions is necessary for translation.
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