Key result
Artificial microRNA targeting the 3D polymerase of foot-and-mouth disease virus significantly reduced progeny virus titre by approximately 2 log10 (99% inhibition) at 24 hours post-infection.
Why the study?
RNA interference using artificial microRNAs is being explored to inhibit foot-and-mouth disease virus replication, but delivery in vivo remains challenging.
Does artificial microRNA targeting 3D polymerase reduce foot-and-mouth disease virus replication in BHK-21 cells?
Does artificial microRNA targeting 3D polymerase reduce foot-and-mouth disease virus replication in BHK-21 cells?
Effect estimate: 99% inhibition
p-value: p=<0.05
Artificial microRNA targeting the 3D polymerase of foot-and-mouth disease virus efficiently inhibits viral replication in vitro, suggesting a potential antiviral strategy.
Warrants exploration of microRNA antivirals for FMDV; leaves open in vivo efficacy and translation.
Foot-and-mouth disease (FMD) is a devastating acute viral disease of livestock with cloven hooves. Among various therapeutic control measures, RNA interference (RNAi) is one of the methods being explored to inhibit FMD virus replication and spread. The RNAi is achieved by short hairpin RNAs or artificial microRNAs (amiRNAs). Utility of amiRNAs as antiviral, targeting conserved regions of the viral genome is gaining importance. However, delivery of miRNA in vivo is still a challenge. In this study, the efficacy of amiRNAs in preventing FMD virus replication in a permissive cell culture system was investigated, by generating stable cell lines expressing amiRNAs targeting three functional regions of the FMD virus (FMDV) genome (IRES, 3B3 and 3D). The results showed that amiRNA targeting 3D polymerase is relatively more efficient. However, expression of multiple microRNAs targeting the three regions did not exhibit additive effect. The data suggest that 3D specific miRNA is a potential valid strategy in developing novel antiviral measures against FMDV infection.
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Basagoudanavar et al. (2019) studied Foot-and-mouth disease virus (FMDV) infection. Artificial microRNA (amiRNA) targeting 3D polymerase vs. Negative control miRNA was evaluated on Progeny virus titre reduction at 24 hours post infection (99% inhibition, p=<0.05). Artificial microRNA targeting the 3D polymerase of foot-and-mouth disease virus significantly reduced progeny virus titre by approximately 2 log10 (99% inhibition) at 24 hours post-infection.
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