July 25, 2008Virology JournalOpen Access

Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA

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Population

Baby hamster kidney (BHK-21) cells and Kunming White suckling mice (2-3 days old, weighing 3-4 g)

Comparison

siRNA-expressing plasmids targeting homogenous… vs Control plasmid or saline injection

Design

Preclinical

Follow-up

Up to 48 hours in vitro; 5-6 days postchallenge in vivo

Authors

ZGZhiru GuoGansu University of Traditional Chinese Medicine

Discussion

Member takes

Implication

Supports preclinical siRNA evaluation for FMDV; leaves open translation beyond suckling mice.

Structured PICO

Population

Baby hamster kidney (BHK-21) cells and Kunming White suckling mice (2-3 days old, weighing 3-4 g)

Intervention

siRNA-expressing plasmids (pSi-FMD2 and pSi-FMD3) targeting homogenous 3D and 2B1 regions of FMDV, administered via transfection in vitro and subcutaneous injection (50-100 ug) in vivo

Comparator

Control plasmid (in vitro) or saline injection (in vivo)

Outcome

Cytopathic effect (CPE) and viral titers (TCID50) in vitro; survival rate after viral challenge in vivosurrogate

siRNA targeting conserved regions of the FMDV genome can partially protect suckling mice from lethal viral challenge and reduce viral replication in vitro.

Limitations

Inhibition effect not as well-defined as in some previous reports
Further research required to determine efficacy for other serotypes

Cite This Study

Zhiru Guo (2008) studied this question.

synapsesocial.com/papers/6a7dd4cea76b9ebabb045717 https://doi.org/10.1186/1743-422x-5-86

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Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Inhibition of foot-and-mouth disease virus replication in vitro and in vivo by small interfering RNA

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider