What is the clinical evidence from this study?

Study design: Other. Population: Foot-and-mouth disease virus (FMDV) infection. Intervention: IRES-specific Dual-miRNA (pmiR242+276) vs. PBS or virus only. Primary outcome: Inhibition of EGFP expression and FMDV replication.

January 6, 2014Virology JournalOpen Access

Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

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Key result

IRES-specific Dual-miRNA significantly inhibited FMDV replication in vitro and delayed death or provided protection in suckling mice challenged with lethal doses of FMDV.

Population

BHK-21 cells, goat fibroblasts, and Kunming White suckling mice challenged with Foot-and-mouth disease virus.

Comparison

IRES-specific microRNAs, specifically Dual-miRNA… vs Negative control and positive control.

Design

Preclinical

Follow-up

up to 7 days

Authors

YCYanyan ChangNanjing Tech University YDYongxi DouLanzhou Veterinary Research Institute HBHuifang BaoLanzhou Veterinary Research Institute

Discussion

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Implication

Hypothesis-generating for miRNA antivirals in FMDV; leaves open validation in livestock models before any translational consideration.

Structured PICO

Population

In vitro cell cultures (BHK-21, goat fibroblasts) and 2-3 day old suckling mice used to evaluate the antiviral effects of IRES-specific miRNAs against foot-and-mouth disease virus.

Intervention

IRES-specific microRNAs (miRNAs), specifically Dual-miRNA (pmiR242 + 276) targeting the internal ribosome entry site of FMDV.

Comparator

Negative control (pmiR-NC plasmid or 1x PBS) and positive control (FMDV infection without miRNA treatment).

Outcome

Inhibition of FMDV replication in vitro and survival of suckling mice challenged with lethal doses of FMDV.surrogate

IRES-specific miRNAs, particularly Dual-miRNA constructs, effectively inhibit FMDV replication in vitro and provide partial to complete protection against lethal FMDV challenge in suckling mice.

Limitations

The Dual-miRNA had no inhibitory effect on the RNA replication of the Mya98 strain of FMDV serotype O in vitro despite its efficacy in vivo.
Different gene silencing efficiencies and expression levels could not guarantee complete inhibition of FMDV replication.

Cite This Study

Chang et al. (2014) studied Foot-and-mouth disease virus (FMDV) infection. IRES-specific Dual-miRNA (pmiR242+276) vs. PBS or virus only was evaluated on Inhibition of EGFP expression and FMDV replication. IRES-specific Dual-miRNA significantly inhibited FMDV replication in vitro and delayed death or provided protection in suckling mice challenged with lethal doses of FMDV.

synapsesocial.com/papers/6a225ccc7d05348b2ab600bc https://doi.org/10.1186/1743-422x-11-1

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Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Multiple microRNAs targeted to internal ribosome entry site against foot-and-mouth disease virus infection in vitro and in vivo

Discussion

Member takes

Implication

Structured PICO

Limitations

Cite This Study

Also Consider

Also Consider