Key result
Nine nucleotides at the 3' end and 80 nucleotides at the 5' end of the HA coding region are sufficient for efficient virion incorporation, enabling the generation of a viable influenza vector stably expressing VSVG and GFP.
Population
In vitro cell lines and influenza A virus models
Comparison
Recombinant influenza A virus constructs with… vs Control constructs lacking HA coding regions…
Design
Preclinical
Authors
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Hypothesis-generating for stable influenza-based gene delivery vectors in animals; leaves open human translation pending safety and efficacy trials.
Identification of specific HA vRNA packaging signals allows for the creation of stable influenza virus-based vectors for foreign gene delivery.
Watanabe et al. (2003) studied Influenza A virus vector development. Recombinant influenza virus vector with HA and NA replaced by VSVG and GFP vs. Wild-type or control constructs was evaluated on Virion incorporation efficiency of HA vRNA and viability of recombinant virus. Nine nucleotides at the 3' end and 80 nucleotides at the 5' end of the HA coding region are sufficient for efficient virion incorporation, enabling the generation of a viable influenza vector stably expressing VSVG and GFP.
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