Key result
Ectopic expression of PKR in cells lacking endogenous PKR demonstrated that its activation and biologic functions are independent of its N-terminal domain.
Population
Cells lacking endogenous PKR
Design
Preclinical
Authors
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PKR N-terminal independence in cell models leaves open its validation in physiologic interferon pathways; further studies needed before any translational consideration.
This study demonstrates that PKR can function and be activated independently of endogenous PKR and its N-terminal domain, highlighting the importance of the third basic domain for its biologic function.
Rivas et al. (1999) studied this question. Ectopic expression of PKR from vaccinia virus recombinants was evaluated on PKR-mediated inhibition of protein synthesis and induction of apoptosis. Ectopic expression of PKR in cells lacking endogenous PKR demonstrated that its activation and biologic functions are independent of its N-terminal domain.
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