Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
September 14, 2006Journal of Virology

Role of RNA Structure and RNA Binding Activity of Foot-and-Mouth Disease Virus 3C Protein in VPg Uridylylation and Virus Replication

View Full Paper
Ask AI
Bookmark
Share

Key result

Specific residues on the surface of the FMDV 3C protein opposite the catalytic site are essential for interaction with cre/bus RNA, VPg uridylylation, and efficient virus replication.

Population

In vitro model of foot-and-mouth disease virus (FMDV) replication components (3B, 3Dpol, 3CD, RNA template)

Design

Preclinical

Authors

ANArabinda NayakIGIan GoodfellowKWKathryn E. Woolaway

Discussion

Loading...

Member takes

Overview

May guide FMDV antiviral design; leaves open in vivo efficacy and therapeutic translation.

Structured PICO

P
Population
In vitro model of foot-and-mouth disease virus (FMDV) replication components (3B, 3Dpol, 3CD, RNA template)
I
Intervention
FMDV 3C protein and specific RNA sequences within the FMDV 5' untranslated region
O
Outcome
VPg uridylylation activity and virus replicationsurrogate

Identifies specific RNA sequences and 3C protein residues essential for VPg uridylylation and FMDV replication in vitro.

Cite This Study

Nayak et al. (2006) studied Foot-and-Mouth Disease Virus (FMDV) replication. FMDV 3C protein mutations and RNA structure modifications vs. Wild-type FMDV 3C protein and RNA was evaluated on VPg uridylylation activity and virus replication. Specific residues on the surface of the FMDV 3C protein opposite the catalytic site are essential for interaction with cre/bus RNA, VPg uridylylation, and efficient virus replication.

synapsesocial.com/papers/6a9450bb5cc4cfd2f72dd101https://doi.org/10.1128/jvi.00561-06
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Sequence Requirements for Viral RNA Replication and VPg Uridylylation Directed by the Internal <i>cis</i> -Acting Replication Element ( <i>cre</i> ) of Human Rhinovirus Type 142002 · 85 citations
  2. 2Crystallization of foot-and-mouth disease virus 3C protease: surface mutagenesis and a novel crystal-optimization strategy2005 · 41 citations
  3. 3Poliovirus <i>cre</i> (2C)-Dependent Synthesis of VPgpUpU Is Required for Positive- but Not Negative-Strand RNA Synthesis2003 · 96 citations
  4. 4Functional Dissection of a Poliovirus <i>cis</i> -Acting Replication Element [PV- <i>cre</i> (2C)]: Analysis of Single- and Dual- <i>cre</i> Viral Genomes and Proteins That Bind Specifically to PV- <i>cre</i> RNA2003 · 83 citations
  5. 5The Foot-and-Mouth Disease Virus <i>cis</i> -Acting Replication Element ( <i>cre</i> ) Can Be Complemented in <i>trans</i> within Infected Cells2003 · 23 citations